Thermoelectric separation copper substrates are usually made of copper and thermoelectric materials. Among them, copper is a metal material with excellent electrical conductivity and is usually used as a conductive part in a circuit; thermoelectric materials are special materials with thermoelectric effects that can generate electrical energy under the action of temperature gradients. Thermoelectric materials are usually composed of two different types of crystals that have opposite thermoelectric effects, one whose resistance increases as the temperature increases (called a "positive Seebeck coefficient") and the other which decreases its resistance as the temperature increases. (called "Seebeck coefficient is negative"). The selection and optimization of this material is crucial to improve the performance of thermoelectric separation copper substrates.